UTechBiotech.org, Kolkata, India

Downstream Processing
       
    Credits: 3 credits
No. of Lectures: 42
  
       
   

Introduction: Introduction to fermentation process, Microbial Growth Kinetics, Media for industrial fermentations, Sterilization, Development of inocula for industrial fermentations, Design of a fermenter, Instrumentation and control, recovery and purification of fermentation products, Effluent Treatment, Fermentation economics.
An overview of bioseparation.  Separation of cells and other insolubles from fermented broth.  Filtration and microfiltration, centrifugation (batch, continuous, basket).  Cell disruption: Physical methods (osmotic shock, grinding with abrasives, solid shear, liquid shear), Chemical methods (alkali, detergents), Enzymatic methods.

Product isolation: Extraction and absorption method, precipitation (ammonium sulphate, Organic solvents, high molecular weight polymers), column chromatography, ultrafiltration. Products polishing: Crystallisation and drying.
       
  Recommended Text  
    1. Principles of Fermentation Technology by Stanier, Whitaker and Hall
2. Biochemical Engineering Fundamentals by JE Bailey and DF Ollis
3. Bioprocess Engineering – Basic Concepts by Shuler and Kargi		  
     
  Lecture Hours Distribution  
    Introduction to fermentation process
Lecture 1
       
    Microbial Growth Kinetics
Lecture 2
       
    Media for industrial fermentations
Lecture 3
       
    Sterilization
Lecture 4
       
    Development of inocula for industrial fermentations
Lecture 5
       
    Design of a fermenter
Lecture 6
       
    Instrumentation and control
Lecture 7
       
    Recovery and purification of fermentation products
Lecture 8
       
    Effluent Treatment
Lecture 9
       
    Fermentation economics
Lecture 10
       
    An overview of bioseparation
Lecture 11
       
    Separation of cells and other insolubles from fermented broth.
Lecture 12
       
    Filtration and microfiltration,
Lecture 13
       
    Centrifugation (batch, continuous, basket).
Lecture 14
       
    Cell disruption: Physical methods (osmotic shock, grinding with abrasives, solid shear, liquid shear),
Lecture 15
       
    Chemical methods (alkali, detergents), Enzymatic methods.
Lecture 16
       
    Product isolation: Extraction and absorption method.
Lecture 17-19
       
    Precipitation (ammonium sulphate, Organic solvents, high molecular weight polymers)
Lecture 20-22
       
    Column chromatography
Lecture 23-26
       
    Ultrafiltration
Lecture 27
       
    Products polishing
Lecture 28
       
    Crystallisation
Lecture 29-30
       
    Drying
Lecture 31
       
   
TOTAL
42 Lectures

 

3 Credit (Theory) means 3 lecture hours each week or 42 lectures per semester.
2 Credit (Lab) means at least 40 hours of lab work per semester.